Simultaneously improving the activity and thermostability of hyperthermophillic pullulanase by modifying the active-site tunnel and surface lysine
Ting Xie, Li Zhou, Laichuang Han, Zhongmei Liu, Wenjing Cui, Zhongyi Cheng, Junling Guo, Yaqin Shen, Zhemin Zhou
Topics & Concepts
ThermostabilityPullulanasePullulanProtein engineeringDirected evolutionChemistryHydrolysisActive siteBiochemistryEnzymePolysaccharideGeneMutantEnzyme Production and CharacterizationPhytase and its ApplicationsEnzyme Catalysis and Immobilization