RNA chain length and stoichiometry govern surface tension and stability of protein-RNA condensates
Rabia Laghmach, Ibraheem Alshareedah, Matthew Pham, Muralikrishna Raju, Priya R. Banerjee, Davit A. Potoyan
Abstract
microscopy measurements and coarse-grained molecular dynamics simulations. We find that crowding and RNA chain length can have a major stabilizing effect on the condensation. We also find that the RNA-protein charge ratio is a crucial variable controlling stability, interfacial properties, and the reentrant phase behavior of RGG-RNA mixtures.
Topics & Concepts
RNABiophysicsChemistryMacromolecular crowdingStoichiometryCrowdingChemical physicsCrystallographyBiologyBiochemistryMacromoleculePhysical chemistryGeneNeuroscienceRNA Research and SplicingRNA modifications and cancerRNA and protein synthesis mechanisms