Single-cell RNA sequencing v1
Klaus H. Kaestner Lab, Suzanne N. Shapira
Abstract
Single-cell RNA sequencing (scRNA-seq) allows for transcriptional profiling of individual cells within a heterogenous sample. This protocol describes a method for performing scRNA-seq using handpicked pancreatic islets from organ donors.
Topics & Concepts
RNARNA-SeqComputational biologyProfiling (computer programming)TranscriptomeBiologyCellGeneComputer scienceGeneticsGene expressionOperating systemPancreatic function and diabetesPancreatic and Hepatic Oncology ResearchSingle-cell and spatial transcriptomics