Intra- and interchromosomal contact mapping reveals the Igh locus has extensive conformational heterogeneity and interacts with B-lineage genes
Olga Mielczarek, Carolyn H. Rogers, Yinxiu Zhan, Louise S. Matheson, Michael J. T. Stubbington, Stefan Schoenfelder, Daniel J. Bolland, Biola M. Javierre, Steven Wingett, Csilla Várnai, Anne Segonds-Pichon, Simon J. Conn, Felix Krueger, Simon Andrews, Peter Fraser, Luca Giorgetti, Anne E. Corcoran
Abstract
genes to its 3' end, reconciling two views of locus organization. Deconvolution of single Igh locus conformations using polymer simulations identifies thousands of different structures. This heterogeneity may underpin the diversity of V(D)J recombination events. All three immunoglobulin loci also participate in a highly specific, developmentally regulated network of interchromosomal interactions with genes encoding B cell-lineage factors. This suggests a model of interchromosomal coordination of B cell development.