Investigating the K+ sensitivity of cellular metabolism by extracellular flux analysis

Abstract

We have recently demonstrated that the activity of hexokinase 2 is dependent on the intracellular potassium ion (K + ) concentration ([K + ]). To analyze the K + dependency of the cell metabolism in cell populations, we used an extracellular flux analyzer to assess oxygen consumption and acidification rates as well-established measures of oxidative- and glycolytic metabolic activities. This protocol describes in detail how a potential K + sensitivity of the cell metabolism can be elucidated by extracellular flux analysis. For complete details on the use and execution of this protocol, please refer to Bischof et al. (2021).

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