Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids
Bankala Krishnarjuna, Thirupathi Ravula, Ayyalusamy Ramamoorthy
Abstract
Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.
Topics & Concepts
MembraneChemistryCytochrome b5Characterization (materials science)Cytochrome cExtraction (chemistry)Membrane proteinBiophysicsCytochromeBiochemistryChromatographyMaterials scienceBiologyNanotechnologyEnzymeMitochondrionLipid Membrane Structure and BehaviorAnalytical Chemistry and SensorsMass Spectrometry Techniques and Applications