Semiflexible Immunobrushes Induce Enhanced T Cell Activation and Expansion
Roel Hammink, Jorieke Weiden, Dion Voerman, Carlijn Popelier, Loek J. Eggermont, Marjolein Schluck, Carl G. Figdor, Martijn Verdoes
Abstract
expansion of activated primary human T cells even at very low numbers of stimulating antibodies compared to rigid beads. Importantly, the immunobrush architecture appeared crucial for this improved T cell activating capacity. Immunobrushes outperform current benchmarks by producing higher numbers of T cells exhibiting a combination of beneficial phenotypic characteristics, such as reduced exhaustion marker expression, high cytokine production, and robust expression of cytotoxic hallmarks. This study indicates that semiflexible immunobrushes have great potential in making T cell-based immunotherapies more effective.