Receptor-associated protein impairs ligand binding to megalin and megalin-dependent endocytic flux in proximal tubule cells
Kimberly R. Long, Youssef Rbaibi, Ossama B. Kashlan, Ora A. Weisz
Abstract
Receptor-associated protein inhibits binding and uptake of all known endogenous ligands by megalin and cubilin receptors via unknown mechanism(s). Here, we took advantage of recently generated knockout cell lines to dissect the effect of this protein on megalin- and cubilin-mediated endocytosis. Our study reveals a novel role for receptor-associated protein in blocking megalin-stimulated endocytic uptake of fluid-phase markers and receptor-bound ligands in proximal tubule cells in addition to its direct effect on ligand binding to megalin receptors.
Topics & Concepts
Endocytic cycleEndocytosisReceptorCell biologyChemistryEndogenyBiochemistryBiologyCellular transport and secretionRetinal Development and DisordersGlycosylation and Glycoproteins Research