Validation of the bag‐mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya
Christine S. Fagnant-Sperati, You Ren, Nicolette A. Zhou, Evans Komen, Benjamin Mwangi, Joanne Hassan, Agnes Chepkurui, Rosemary Nzunza, James Nyangao, Walda B. van Zyl, M. Wolfaardt, Peter N. Matsapola, Fhatuwani B. Ngwana, Stacey Jeffries Miles, Angela Coulliette-Salmond, Silvia Peñaranda, Everardo Vega, Jeffry H. Shirai, Alexandra L. Kossik, Nicola K. Beck, D.S. Boyle, Cara C. Burns, Maureen B. Taylor, Peter Borus, John Scott Meschke
Abstract
AIMS: This study compared the bag-mediated filtration system (BMFS) and standard WHO two-phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin-like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016. METHODS AND RESULTS: ). CONCLUSIONS: Poliovirus was detected at higher rates with the BMFS, a method that includes a secondary concentration step, than using the standard WHO two-phase method. SL2 disappearance from the environment was commensurate with OPV2 withdrawal. SIGNIFICANCE AND IMPACT OF THE STUDY: The BMFS offers comparable or improved PV detection under the conditions in this study, relative to the two-phase method.