Modulation of hepatitis B virus pregenomic RNA stability and splicing by histone deacetylase 5 enhances viral biosynthesis
Taha Y. Taha, Varada Anirudhan, Umaporn Limothai, Daniel D. Loeb, Pavel A. Petukhov, A. McLachlan
Abstract
Hepatitis B virus (HBV) is a worldwide health problem without curative treatments. Investigation of the regulation of HBV biosynthesis by class I and II histone deacetylases (HDACs) demonstrated that catalytically active HDAC5 upregulates HBV biosynthesis. HDAC5 expression increased both the stability and splicing of the HBV 3.5 kb RNA without altering the translational efficiency of the viral pregenomic or spliced 2.2 kb RNAs. Together, these observations point to a broader role of HDAC5 in regulating RNA splicing and transcript stability while specifically identifying a potentially novel approach toward antiviral HBV therapeutic development.
Topics & Concepts
RNA splicingHepatitis B virusHistone deacetylaseHistone deacetylase 5VirologyRNAHistoneBiologyVirusChemistryGeneBiochemistryHepatitis B Virus StudiesRNA Interference and Gene DeliveryHIV Research and Treatment