Pseudomonas aeruginosa Oligoribonuclease Controls Tolerance to Polymyxin B by Regulating Pel Exopolysaccharide Production
Baopeng Yang, Yujun Jiang, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu
Abstract
increased the bacterial surface bound polymyxin B. Deletion of the Pel synthesis genes or treatment with a Pel hydrolase reduced the surface bound polymyxin B and bacterial survival. We further demonstrated that Pel binds to extracellular DNA (eDNA), which traps polymyxin B and thus protects the bacterial cells. Collectively, our results revealed a novel defense mechanism against polymyxin in P. aeruginosa.
Topics & Concepts
PolymyxinPolymyxin BPseudomonas aeruginosaMicrobiologyBacterial outer membraneMutantLipopolysaccharideBacteriaAminoglycosideBiologyExtracellularPseudomonadaceaeChemistryColistinKanamycinPseudomonadalesIntracellularAntibioticsEnzymePilusMultidrug toleranceAntibacterial agentBacterial cell structureLipid APseudomonasOperonAntibiotic Resistance in BacteriaImmune Response and InflammationAntimicrobial agents and applications