Biosynthetic Gene Cluster for Asperterpenols A and B and the Cyclization Mechanism of Asperterpenol A Synthase
Zhiyang Quan, Jeroen S. Dickschat
Abstract
The biosynthetic gene cluster for the sesterterpenoids asperterpenols A and B from Aspergillus calidoustus CBS121601 is described. The cluster contains two genes, acldAS for a chimeric sesterterpene synthase and acldA-P450 for a cytochrome P450 monooxygenase. The cyclization mechanism of the asperterpenol A synthase, catalyzing the formation of a unique sesterterpene with a 6/6/8/5 tetracyclic ring system, was studied using isotopically labeled terpene precursors.
Topics & Concepts
ChemistryGene clusterATP synthaseTerpeneStereochemistryMonooxygenaseCytochrome P450Ring (chemistry)CytochromeEnzymeGeneMechanism (biology)BiochemistryOrganic chemistryEpistemologyPhilosophyPlant biochemistry and biosynthesisMicrobial Natural Products and BiosynthesisNatural product bioactivities and synthesis