Improvement of B Cell Responses by an HIV-1 Amphiphilic Polymer Nanovaccine
Xiaoqian Xin, Yifeng Liu, Lei Guo, Hui Wang, Daiqiang Lu, Yaotian Chang, Mingming Wan, Yong Zhang, Yaming Shan, Qiao Zhang, Xiaowen Liu, Feng Gao
Abstract
The human immunodeficiency virus (HIV) has infected over 84 million people since its discovery and is a huge threat to human health. While an HIV vaccine is urgently needed to curb this devastating pandemic, it has been notoriously difficult to develop, partly due to the extraordinary high level of genetic variation of HIV. We designed a new HIV-1 envelope glycoprotein nanoparticle (Env/NP) vaccine using amphiphilic polymers. The Env/NP vaccine induced more potent and broader neutralizing activities against multiple HIV-1 subtypes. Moreover, it elicits similar neutralizing antibody responses after the storage at -80 °C, 4 °C or room temperature post lyophilization. These results demonstrate that the new Env/NP vaccine not only improves the HIV vaccine immune responses but also is stable under different storage conditions. This new nanovaccine approach can readily apply to other protein-based vaccines.