Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors
Katherine Chan, Adrian Granda Farias, Hunsang Lee, Furkan Guvenc, Patricia Mero, Kevin R. Brown, Henry N. Ward, Maximilian Billmann, Kamaldeep Aulakh, Audrey Astori, Shahan Haider, Edyta Marcon, Ulrich Braunschweig, Shuye Pu, Andrea Habsid, Amy H.Y. Tong, Natasha Christie-Holmes, Patrick Budylowski, Ayoob Ghalami, Samira Mubareka, Finlay Maguire, Arinjay Banerjee, Karen Mossman, Jack Greenblatt, Scott D. Gray‐Owen, Brian Raught, Benjamin J. Blencowe, Mikko Taipale, Chad L. Myers, Jason Moffat
Abstract
in Calu-3 cells had the strongest impact in preventing SARS-CoV-2 infection when perturbed.
Topics & Concepts
BiologyCRISPRHEK 293 cellsViral replicationGenetic screenCell cultureViral entryGeneChromatinHost factorGeneticsDruggabilityComputational biologyVirologyPhenotypeSARS-CoV-2 and COVID-19 ResearchCRISPR and Genetic EngineeringCOVID-19 Clinical Research Studies