From peptides to proteins: coiled-coil tetramers to single-chain 4-helix bundles
Elise A. Naudin, Katherine I. Albanese, Abigail J. Smith, B. Mylemans, Emily G. Baker, Orion D. Weiner, David Andrews, Natalie J. Tigue, Nigel J. Savery, Derek N. Woolfson
Abstract
. All of the assembled designs are confirmed in aqueous solution using biophysical methods, and ultimately by determining high-resolution X-ray crystal structures. Our route from peptides to proteins provides an understanding of the role of each residue in each design.
Topics & Concepts
Coiled coilHelix (gastropod)Single chainChemistryChain (unit)Helix bundleCrystallographyStereochemistryBiophysicsProtein structureBiochemistryBiologyGeneticsPhysicsAstronomySnailAntibodyEcologyGlycosylation and Glycoproteins ResearchRNA and protein synthesis mechanismsChemical Synthesis and Analysis