A new urinary exosome enrichment method by a combination of ultrafiltration and TiO<sub>2</sub> nanoparticles
Xiaochao Xiang, Fulin Guan, Fenglong Jiao, Hang Li, Wanjun Zhang, Yangjun Zhang, Weijie Qin
Abstract
The workflow of separation and enrichment of exosomes by ultrafiltration–TiO<sub>2</sub>. We proposed a new strategy for facile exosome isolation from human urine by utilizing the ultrafiltration technique and TiO<sub>2</sub>, which can significantly reduce urine volumes, increase exposure of the material to exosomes in urine and obtain high purity exosomes.
Topics & Concepts
Ultrafiltration (renal)ExosomeNanoparticleMicrovesiclesChromatographyChemistryUrinary systemNanotechnologyMaterials scienceBiochemistryMedicineInternal medicineGenemicroRNAExtracellular vesicles in disease