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Drug-Resistant Characteristics, Genetic Diversity, and Transmission Dynamics of Rifampicin-Resistant Mycobacterium tuberculosis in Hunan, China, Revealed by Whole-Genome Sequencing

Wencong He, Yun‐Hong Tan, Chunfa Liu, Yiting Wang, Ping He, Zexuan Song, Dongxin Liu, Huiwen Zheng, Aijing Ma, Bing Zhao, Xichao Ou, Hui Xia, Shengfen Wang, Yanlin Zhao

2022Microbiology Spectrum43 citationsDOIOpen Access PDF

Abstract

Comprehensive information such as genetic background and drug-resistant profile of MTB strains could help to tailor public interventions. However, these data are limited in Hunan province, one of the provinces with high-TB burden in China. So, this study aimed to provide us with deep insight into the molecular epidemiology of RR-TB isolates circulating in Hunan province by combining phenotypic drug susceptibility testing and whole-genome sequencing. To our knowledge, this is the first study to use whole-genome sequencing data of RR-TB strains spanning more than 5 years for molecular epidemiology analysis in Hunan province, which allows us to identify genetic background information and clustered strains more accurately. Our study revealed the complexity of RR-TB strains circulating in Hunan province with complex additional drug-resistant profile and relatively higher clustering rates. Comprehensive information based on WGS should be used to guide the design of treatment regimens and tailor public interventions.

Topics & Concepts

Lineage (genetic)TuberculosisRifampicinMycobacterium tuberculosisDrug resistancePyrazinamideBiologyTransmission (telecommunications)Genetic diversityWhole genome sequencingAntibiotic resistanceVirologyAntibioticsGeneticsGenomeMedicineGenePopulationEnvironmental healthPathologyElectrical engineeringEngineeringTuberculosis Research and EpidemiologyMycobacterium research and diagnosisPneumocystis jirovecii pneumonia detection and treatment
Drug-Resistant Characteristics, Genetic Diversity, and Transmission Dynamics of Rifampicin-Resistant Mycobacterium tuberculosis in Hunan, China, Revealed by Whole-Genome Sequencing | Litcius