Properties and Printability of the Synthesized Hydrogel Based on GelMA
Nadezhda V. Arguchinskaya, Елена Исаева, Anastas Kisel, Е. Е. Бекетов, Tatiana S. Lagoda, Denis Baranovskii, Nina D. Yakovleva, Grigory Demyashkin, Liudmila N. Komarova, Svetlana O. Astakhina, Nikolai E. Shubin, Peter Shegay, С. А. Иванов, А. Д. Каприн
Abstract
Gelatin methacryloyl (GelMA) has recently attracted increasing attention. Unlike other hydrogels, it allows for the adjustment of the mechanical properties using such factors as degree of functionalization, concentration, and photocrosslinking parameters. In this study, GelMA with a high degree of substitution (82.75 ± 7.09%) was synthesized, and its suitability for extrusion printing, cytocompatibility, and biocompatibility was studied. Satisfactory printing quality was demonstrated with the 15% concentration hydrogel. The high degree of functionalization led to a decrease in the ability of human adipose-derived stem cells (ADSCs) to adhere to the GelMA surface. During the first 3 days after sowing, proliferation was observed. Degradation in animals after subcutaneous implantation was slowed down.