Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers
Xavier Busuttil-Crellin, Conor McCafferty, Suelyn Van Den Helm, Hui Ping Yaw, Paul Monagle, Matthew D. Linden, Vera Ignjatović
Abstract
Flow cytometry is a valuable tool in determining the phenotype and function of platelets accurately. The emergence of platelet flow cytometry in recent years provides an attractive alternative to other platelet analytical techniques, with advantages such as requiring small volumes and being highly sensitive to minimal changes in receptor function and expression. Here we present a methodical approach encompassing the stages in the development and optimization of platelet flow cytometry panels based on our extensive experience in this area.
Topics & Concepts
Flow cytometryPlateletCytometryPhenotypeFunction (biology)Flow (mathematics)Platelet activationWhole bloodBiomedical engineeringComputer scienceImmunologyMedicineBiologyCell biologyMathematicsBiochemistryGeometryGenePlatelet Disorders and TreatmentsImmune Cell Function and InteractionSingle-cell and spatial transcriptomics