Development of an insect cell-based adeno-associated virus packaging cell line employing advanced Rep gene expression control system
Fiona Moreno, Florence Lip, Hugo Rojas, Anggakusuma
Abstract
gene control system, ensuring stable and appropriate Rep expression. This platform produces potent and high-yield AAV particles and demonstrates potential for scale up.
Topics & Concepts
BiologyPlasmidTransgeneBaculoviridaeGeneEnhancerExpression cassetteCell cultureHomologous recombinationRecombinant DNAVector (molecular biology)Gene expressionCell biologyGeneticsSpodopteraViral Infectious Diseases and Gene Expression in InsectsVirus-based gene therapy researchCRISPR and Genetic Engineering