Development of CRISPR/Cas12b-Based Multiple Cross Displacement Amplification Technique for the Detection of <i>Mycobacterium tuberculosis</i> Complex in Clinical Settings
Xinggui Yang, Junfei Huang, Yijiang Chen, Xia Ying, Qinqin Tan, Xu Chen, Xiaoyan Zeng, Shiguang Lei, Yi Wang, Shijun Li
Abstract
sequence to detect MTC pathogens. These results demonstrated that the CRISPR-MCDA assay developed in this study was a rapid, ultrasensitive, highly specific, and readily available method which can be used as a valuable diagnostic tool for MTC infection in clinical settings.
Topics & Concepts
Mycobacterium tuberculosisCRISPRTuberculosisDisplacement (psychology)Mycobacterium tuberculosis complexBiologyComputational biologyComputer scienceGeneticsMedicinePsychologyGenePathologyPsychotherapistCRISPR and Genetic EngineeringTuberculosis Research and EpidemiologyCytomegalovirus and herpesvirus research