Nitrogenase Cofactor Maturase NifB Isolated from Transgenic Rice is Active in FeMo-co Synthesis
Wenshu He, Stefan Burén, Can Baysal, Xi Jiang, Teresa Capell, Paul Christou, Luis M. Rubio
Abstract
, and the purified proteins were functional in the in vitro FeMo-co synthesis assay. We thus report NifB protein expression and purification from an engineered staple crop, representing a first step in the biosynthesis of a functional NifDK complex, as required for independent biological nitrogen fixation in cereals.
Topics & Concepts
NitrogenaseBiogenesisCofactorBiochemistryBiosynthesisNitrogen fixationAzotobacter vinelandiiBiologyArchaeaChemistryEnzymeGeneBacteriaGeneticsMetalloenzymes and iron-sulfur proteinsElectrocatalysts for Energy ConversionCatalysis for Biomass Conversion