The Arg-293 of Cryptochrome1 is responsible for the allosteric regulation of CLOCK-CRY1 binding in circadian rhythm
Şeref Gül, Cihan Aydın, Onur Özcan, Berke Gurkan, Saliha Sürme, İbrahim Barış, İbrahim Halil Kavaklı
Abstract
double knockout mouse embryonic fibroblast cell line. Moreover, the variant displayed reduced repressor activity on BMAL1/CLOCK driven transcription, which is explained by reduced affinity to BMAL1/CLOCK in the absence of PER2 compared with CRY1. Molecular dynamics simulations revealed that the p.Arg293His CRY1 variant altered a communication pathway between Arg-293 and the serine loop by reducing its dynamicity. Collectively, this study provides direct evidence that allosterism in CRY1 is critical for the regulation of circadian rhythm.
Topics & Concepts
CryptochromeRepressorCircadian clockBiologyPER2Circadian rhythmTranscription (linguistics)Cell biologyTranscription factorGeneticsAllosteric regulationTranscriptional regulationCLOCKGeneReceptorNeuroscienceLinguisticsPhilosophyCircadian rhythm and melatoninLight effects on plantsPlant Molecular Biology Research