A study on the effects of tumor-derived exosomes on hepatoma cells and hepatocytes by atomic force microscopy
Tuoyu Ju, Shuwei Wang, Jiajia Wang, Fan Yang, Zhengxun Song, Hongmei Xu, Yujuan Chen, Jingran Zhang, Zuobin Wang
Abstract
, respectively. With an increase in time and concentration, the proliferation effect was more significant. On comparing the mechanical properties of the three types of cells (HCC-LM3, SMMC-7721 and HL-7702 cells), the degradation degree and migration ability of the cells were from high to low in the above order. In turn, the surface roughness of the cells decreased, and adhesion and elastic modulus increased. With an increase in treatment time, surface roughness increased, while adhesion and elastic modulus decreased. These suggested that the HCC-LM3-exos could change the mechanical properties of cells, leading to their deterioration, and enhance their migration and invasion ability. In this paper, the effects of exosomes were analyzed from the perspective of the physical parameters of cells, which provide a new idea to study cancer metastasis and prognosis.