Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe
Jintao Zhao, Yuan Qu, Hao Gao, Miao Zhong, Xinming Li, Fang Zhang, Yaxiong Chen, Lu Gan, Guodong Hu, Hong Zhang, Shengxiang Zhang, Jianguo Fang
Abstract
Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo. With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke.
Topics & Concepts
FluorescenceThioredoxin reductaseTwo-photon excitation microscopyIn vivoPhotonChemistryBiophysicsPhotochemistryBiochemistryBiologyThioredoxinPhysicsGeneticsEnzymeOpticsRedox biology and oxidative stressNitric Oxide and Endothelin EffectsSulfur Compounds in Biology