3D-printed microfluidic device for high-throughput production of lipid nanoparticles incorporating SARS-CoV-2 spike protein mRNA
Wanzhen Lin, William Kristian Vu Bostic, Noah Malmstadt
Abstract
) of LNPs compared to commercial multi-thousand-dollar micromixers. The device produced LNPs of diameter less than 90 nm, with low polydispersity (2-8%) and high mRNA encapsulation efficiency (>90%). The 3D-printed device provides a cost-effective and easily prepared solution for high-throughput LNP production.
Topics & Concepts
MicrofluidicsSpike (software development)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Messenger RNASpike ProteinCoronavirus disease 2019 (COVID-19)Nanotechnology2019-20 coronavirus outbreakThroughputNanoparticleMaterials scienceChemistryVirologyBiologyComputer scienceMedicineBiochemistryGeneTelecommunicationsWirelessOutbreakPathologySoftware engineeringInfectious disease (medical specialty)DiseaseInnovative Microfluidic and Catalytic Techniques Innovation3D Printing in Biomedical ResearchSARS-CoV-2 and COVID-19 Research