Introduction to Single-Cell DNA Methylation Profiling Methods
Jongseong Ahn, Sunghoon Heo, Jihyun Lee, Duhee Bang
Abstract
DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DNA-methylation profiling methods, including experimental strategies and approaches to computational data analysis. Furthermore, the blind spots of the basic analysis and recent alternatives are briefly described. In addition, we introduce well-known applications and discuss future development.
Topics & Concepts
DNA methylationEpigeneticsComputational biologyBiologyMethylationDNAEpigenomicsProfiling (computer programming)GeneticsGeneGene expressionComputer scienceOperating systemEpigenetics and DNA MethylationSingle-cell and spatial transcriptomicsOpportunistic and Delay-Tolerant Networks