The effect of different tobacco tar levels on DNA damage in cigarette smoking subjects
Congcong Zhao, Yuanchen Xie, Xiaoshan Zhou, Qiao Zhang, Na Wang
Abstract
Abstract Objective To explore the genetic damage caused by different tar levels in the human body. Methods The subjects were divided into high, medium and low (12 mg, 8 mg, 5 mg) tar groups according to the tar levels. Nonsmoking populations served as a control group. 2 ml of peripheral blood was collected on the 10th day after morning fasting. Oxidative and genetic toxicological damage indicators were analysed with enzyme-linked immunosorbent assay, cytokinesis-block micronucleus assay in human lymphocyte and single cell gel electrophoresis. Results The distribution of hOGG1 concentration was significantly different within all groups, P < 0.01. The concentrations of cotinine, 8-OHdG and Rap-2b were significantly differences between control and medium tar group, control and high tar group, low and medium tar group and low and high tar group, respectively, P < 0.05. The level of PAH-DNA adducts was not significantly changed in the middle tar group and high tar group, P > 0.05. The level of CRP was significantly changed between control and high tar group, low and high tar group and medium and high tar group, respectively, P < 0.0001. The rate of comet tailing was significantly different between all groups. The rate of micronucleus cells was not significantly different between all groups. Conclusions The increase of tar content could increase the DNA damage to a certain extent, so the intake of tar content should be monitored.