Development of optimized protocol for culturing African swine fever virus field isolates in MA104 cells.
Hyeok-Il Kwon, Duy Tien, Hung Van Vo, Seung‐Chul Lee, Min Ho Kim, Dung Thi Thuy Nguyen, Tan Minh Tran, Quang Tin Vinh Le, Tram Thi Ngoc Ngo, Nam Minh Nguyen, Joo Young Lee, Toan Tat Nguyen
Abstract
= 0.062) in ASFV growth between infectious media A and B. A model was developed to enhance ASFV replication through adaptation to MA104 cells. The lack of mutation at the genetic segments encoding p72, p54, p30, and the central hypervariable region (CVR) in serial culture passages is important in increasing the probability of maintaining immunogenicity when developing a vaccine candidate.
Topics & Concepts
African swine fever virusVirologyBiologyVirusViral replicationAnimal Disease Management and EpidemiologyVector-Borne Animal DiseasesViral Infections and Immunology Research