Construction and application of an efficient dual-base editing platform for <i>Bacillus subtilis</i> evolution employing programmable base conversion
Wenliang Hao, Wenjing Cui, Feiya Suo, Laichuang Han, Zhongyi Cheng, Zhemin Zhou
Abstract
through directed evolution of the subunit of lantibiotic ATP-binding cassette. Therefore, our work provides a portable and programmable genomic diversification platform, which is promising to expedite the fabrication of high-performance and robust bacterial chassis used in the development of biomanufacturing and biopharmaceuticals.
Topics & Concepts
Bacillus subtilisBase (topology)Dual (grammatical number)Computer scienceChemistryComputer architectureComputational biologyBiologyGeneticsMathematicsBacteriaArtLiteratureMathematical analysisCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsBacterial Genetics and Biotechnology