Engineering ketoreductases for the enantioselective synthesis of chiral alcohols
Qiao Li, Zhiyuan Luo, Haomin Chen, Pengfei Zhang, Anming Wang, Roger A. Sheldon
Abstract
The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key intermediates in, for example, pharmaceuticals synthesis. The application of sophisticated protein engineering and enzyme immobilisation techniques to increase industrial viability are discussed.
Topics & Concepts
Enantioselective synthesisChemistryCombinatorial chemistryBiocatalysisReduction (mathematics)Organic chemistryCatalysisMathematicsReaction mechanismGeometryEnzyme Catalysis and ImmobilizationMetabolism and Genetic DisordersPharmacogenetics and Drug Metabolism