A protocol for human bone marrow adipocyte isolation and purification
Camille Attané, David Estève, Mohamed Moutahir, Nicolas Reina, Catherine Muller
Abstract
Primary human bone marrow adipocytes (BM-Ads) display a specific metabolism that is not recapitulated by in vitro differentiated bone marrow mesenchymal stromal cells. These findings highlight the need for using primary BM-Ads in studies of the metabolic impact of BM-Ads on surrounding cells. Here, we present a protocol for isolating human BM-Ads from bone marrow aspirates and verifying adipocyte suspension purity. These isolated and purified BM-Ads can be used for functional assays or frozen for molecular analyses. For complete details on the use and execution of this protocol, please refer to Attane et al. (2020).
Topics & Concepts
Bone marrowStromal cellHuman boneAdipocyteMesenchymal stem cellIn vitroBiologyCell biologyPathologyChemistryMolecular biologyImmunologyAdipose tissueCancer researchMedicineBiochemistryAdipose Tissue and MetabolismHematological disorders and diagnosticsMesenchymal stem cell research