Microtensile Bond Strength of Composite Resin Following the Use of Bromelain and Papain as Deproteinizing Agents on Etched Dentin: An In Vitro Study
Roopa R Nadig, Ranjini M Aswathanarayana, Mohd Sibghatullah Khatib, Swapna V Devarasanahalli, Ashwath H Venkateswara
Abstract
AIM AND OBJECTIVES: The aim of this study was to evaluate and compare the deproteinizing effect of sodium hypochlorite, bromelain, and papain on microtensile bond strength of composite resin to etched dentin. MATERIALS AND METHODS: = 20) based on the method of dentin deproteinization. Group I: only etching; group II: deproteinized with 5.25% sodium hypochlorite for 1 minute; group III: deproteinized with 8% bromelain enzyme for 1 minute; and group IV: deproteinized with 8% papain enzyme for 1 minute. All the samples were washed off with distilled water to remove deproteinizing agents. Sample surfaces were blot dried and bonding of the dentin surface was performed and restored with light cure bulk fill composite. Samples were stored in distilled water (37°C/24 hours) and thermocycled. Then, the teeth were longitudinally sectioned and individually fixed to a sectioning block using acrylic resin. The block was mounted on hard tissue microtome and sectioned to get one to three slabs of 1 mm thick sections. The beam was then attached to a custom-made jig using screws subjected to the Instron universal testing machine. A tensile load was applied at a crosshead speed of 0.5 mm/minute until the beam fractured. RESULTS: = 0.20). CONCLUSION: study, the following conclusions were drawn. The microtensile bond strength of dentine tested in various deproteinizing agents is as follows: 8% bromelain > 8% papain > 5.25% NaOCl > control group. Naturally occurring deproteinizing agents, such as bromelain and papain, used in this study have resulted in greater bond strength values when compared to that of traditionally used chemical agent such as NaOCl. HOW TO CITE THIS ARTICLE: Study. Int J Clin Pediatr Dent 2020;13(1):43-47.