Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase
Li Fan, Wenqing Zhang, Josephine Rybchuk, Yu Luo, Wei Xiao
Abstract
It is known that budding yeast PCNA sumoylation serves as a ligand to recruit a DNA helicase Srs2 through its tandem receptor motifs that prevent unwanted homologous recombination (HR) at replication forks, a process known as salvage HR. This study reveals detailed molecular mechanisms, in which constitutive PCNA-PIP interaction has been adapted to a regulatory event. Since both PCNA and Srs2 are highly conserved in eukaryotes, from yeast to human, this study may shed light to investigation of similar regulatory mechanisms.
Topics & Concepts
Proliferating cell nuclear antigenHelicaseDNA damageSUMO proteinDNA repairBiologyCell biologyMolecular biologyHomologous recombinationUbiquitinSaccharomyces cerevisiaeDNAChemistryGeneticsYeastGeneRNADNA Repair MechanismsFungal and yeast genetics researchCRISPR and Genetic Engineering