A microfluidic electrophoretic dual dynamic staining method for the identification and relative quantitation of dsRNA contaminants in mRNA vaccines
Adriana Coll De Peña, Nina Li, Matei Vaduva, Lloyd Bwanali, Anubhav Tripathi
Abstract
. Based on these estimated values, our method allows for the detection of dsRNA contaminants present in percentages as low as 0.14-0.59% compared to the total mRNA concentration. Here, we discuss the molecular mechanism of the dynamic staining behavior of dsRNA and mRNA for two different stains. We believe our method will accelerate the mRNA vaccine development from initial development to quality control workflows.
Topics & Concepts
VirologyRNA silencingBiologyMessenger RNAPathogenMicrofluidicsStainingIdentification (biology)RNAMicrobiologyRNA interferenceNanotechnologyGeneGeneticsMaterials scienceBotanyRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesExtracellular vesicles in disease