Inhibitory Effect of Honeysuckle (Lonicera japonica Thunb.) Extract on the Melanosis and Quality Deterioration of Pacific White Shrimp (Litopenaeus vannamei) During Cold Storage
Ouyang Zheng, Huijie Chen, Xiaoye Jia, Yamei Liu, Qinxiu Sun, Zefu Wang, Shucheng Liu
Abstract
The effect of Lonicera japonica Thunb. (LJT) extract on melanosis and quality deterioration of Pacific white shrimp (Litopenaeus vannamei) during cold storage was evaluated, using 4-hexylresorcinol (4-HR), a commercial melanosis inhibitor, as a positive control. The results showed that LJT inhibited polyphenol oxidase (PPO) activity and complexed copper ions in a dose-dependent manner (LJT < 16 mg/mL), indicating that LJT has the potential to inhibit shrimp melanization. Visual image and grayscale value analysis revealed that LJT and 4-HR effectively alleviated melanosis and delayed deterioration of shrimp quality stored at 4 °C, with LJT exhibiting a superior effect. LJT samples displayed a lower total number of colonies, total volatile base nitrogen value, and thiobarbituric reactive substance value than the others (p < 0.05), suggesting that LJT exerted the most potent inhibition on microbial growth and lipid oxidation in shrimps during cold storage. Consequently, LJT effectively retarded the melanosis and quality deterioration of shrimps.