Ultrasound-Assisted extraction and purification of polysaccharides from Boschniakia rossica: Structural Characterization and antioxidant potential
Minghui Yang, Xingfan Li, Xinyi Du, Falin Li, Tianqi Wang, Yanyan Gao, Jia Liu, Xiongfei Luo, Xiaorui Guo, Zhonghua Tang
Abstract
Modern pharmacological investigations have shown that polysaccharides extracted from Boschniakia rossica showcase a range of biological effects. This study identified 260 primary metabolites, with carbohydrate compounds and their derivatives accounting for 80 types. We employed ultrasonic-assisted enzyme extraction (UAEE) to optimize extraction parameters, achieving a crude polysaccharide yield of 13.67%, which is 1.52 times and 1.17 times higher than ultrasonic extraction (UAE) and enzyme extraction (EAE). Additionally, we isolated four distinct purified polysaccharides (BRPS-0, BRPS-1, BRPS-2, BRPS-3), with relative molecular weights of 1569, 2815, and 4572, while BRPS-3 displayed structural complexity. The scanning electron microscope (SEM) revealed that BRPS-0 exhibited distinct crystalline particles, while BRPS-3 displayed an ordered crystal structure. Notably, BRPS-0 demonstrated relatively stable antioxidant activity due to its low molecular weight, high phenolic and sugar content, crystalline microstructure, and abundant α- and β-pyranose configurations, including galacturonic acid, glucose, galactose, and arabinose. This research provides a foundational theory for the comprehensive use of Boschniakia rossica resources and supports its implementation in modern medicine and functional foods.