Comparison of the BioFire Joint Infection Panel to 16S Ribosomal RNA Gene-Based Targeted Metagenomic Sequencing for Testing Synovial Fluid from Patients with Knee Arthroplasty Failure
Marisa Ann Azad, Matthew J. Wolf, Angela P. Strasburg, Matthew L. Daniels, Jordan C. Starkey, Alexander D. Donadio, Matthew P. Abdel, Kerryl E. Greenwood‐Quaintance, Robin Patel
Abstract
< 0.001). JI panel and tMGS overall specificities were 100% (16/16; 95% CI, 81 to 100%) and 94% (15/16; 95% CI, 72 to 99%), respectively. While the clinical sensitivity of the JI panel was excellent for on-panel microorganisms, overall sensitivity for PJI diagnosis was low due to the absence of Staphylococcus epidermidis, a common causative pathogen of PJI, on the panel. A PJI diagnostic algorithm for the use of both molecular tests is proposed.
Topics & Concepts
MedicineSynovial fluidPeriprostheticMetagenomicsInternal medicineConfidence intervalArthroplastyPathologySurgeryOsteoarthritisGeneBiologyAlternative medicineBiochemistryOrthopedic Infections and TreatmentsOrthopaedic implants and arthroplastyTotal Knee Arthroplasty Outcomes