Polymeric biomaterials maintained the esthetic efficacy and reduced the cytotoxicity of in‐office dental bleaching
Uxua Ortecho Zuta, Carla Caroline de Oliveira Duque, Rafael Antônio de Oliveira Ribeiro, Maria Luísa Leite, Diana Gabriela Soares, Josimeri Hebling, André Luíz Fraga Briso, Carlos Alberto de Souza Costa
Abstract
Abstract Evaluate the kinetics of hydrogen peroxide (H 2 O 2 ) degradation, esthetic efficacy and cytotoxicity of a bleaching gel with 35%H 2 O 2 applied on enamel previously covered or not with polymeric nanofibrillar scaffold (SNan), polymeric primer catalyst (PPol), and both. Standardized enamel/dentin discs (n = 128) obtained from bovine teeth were adapted to pulp chambers. After covering enamel with the polymeric products, the bleaching gel was applied for 45 min, establishing the following groups: G1: no treatment (negative control); G2: 35%H 2 O 2 (positive control); G3: SNan; G4: PPol; G5: SNan + PPol; G6: SNan + 35%H 2 O 2 ; G7: PPol + 35%H 2 O 2 ; G8: SNan + PPol + 35%H 2 O 2 . The kinetics of H 2 O 2 degradation (n = 8), bleaching efficacy (ΔE/ΔWI; n = 8), trans‐amelodentinal cytotoxicity (n = 8), and cell morphology (n = 4) were assessed (ANOVA/Tukey test; p < 0.05). Greater H 2 O 2 degradation occurred in G7 and G8. Bleaching efficacy (ΔE) was higher in G6, G7, and G8 in comparison with G2 ( p < 0.05). However, no difference was observed for ΔWI ( p > 0.05). G8 presented the lower level of trans‐amelodentinal diffusion of H 2 O 2 , oxidative stress, and toxicity to the MDPC‐23 cells ( p < 0.05). Polymeric biomaterials increased the kinetics of H 2 O 2 decomposition, as well as maintained the esthetic efficacy and minimized the cytotoxicity caused by a bleaching gel with 35%H 2 O 2 . Clinical Significance Application of a bleaching gel with 35%H 2 O 2 on enamel previously covered by polymeric biomaterials maintains the esthetic efficacy and reduces the cytotoxicity caused by a single session of in‐office dental bleaching.