Antiviral effects of Bovine antimicrobial peptide against TGEV <i>in vivo</i> and <i>in vitro</i>
Xiuli Liang, Xiaojun Zhang, Kaiqi Lian, Tian Xiuhua, Mingliang Zhang, Shiqiong Wang, Cheng Chen, Cunxi Nie, Yun Pan, Fangfang Han, Zhanyong Wei, Wenju Zhang
Abstract
BACKGROUND: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. OBJECTIVES: experiments. METHODS: ). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. RESULTS: of 62.5 μg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 μg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. CONCLUSIONS: .