Intron-targeted mutagenesis reveals roles for Dscam1 RNA pairing architecture-driven splicing bias in neuronal wiring
Weiling Hong, Jian Zhang, Haiyang Dong, Yang Shi, Hongru Ma, Fengyan Zhou, Bingbing Xu, Ying Fu, Shixin Zhang, Shouqing Hou, Guo Li, Yandan Wu, Shawn Chen, Xiaohua Zhu, Wendong You, Feng Shi, Xiaofeng Yang, Zhefeng Gong, Jianhua Huang, Yongfeng Jin
Abstract
exhibits remarkable MB defects, suggesting a variable domain-specific requirement for isoform bias. Importantly, changes in isoform bias cause axonal defects but do not influence the self-avoidance of axonal branches. We conclude that, in contrast to the isoform number that provides the molecular basis for neurite self-avoidance, isoform bias may play a role in MB axonal wiring by influencing non-repulsive signaling.
Topics & Concepts
RNA splicingIntronPairingRNABiologyRNA-binding proteinMutagenesisGeneticsComputational biologyCell biologyMutationGenePhysicsQuantum mechanicsSuperconductivityRNA Research and SplicingRNA and protein synthesis mechanismsRNA regulation and disease