FLAVONOIDS CONSTITUENT ANALYSIS AND CELL CYCLE INHIBITION ACTIVITY OF ETHYLACETATE EXTRACT OF Vernonia amygdalina Delile. LEAVES ON LUNG CANCER CELL LINE
Poppy Anjelisa Zaitun Hasibuan, Delfitri Munir, Dian Ratna Pertiwi, D. Satria, Muhammad Fauzan Lubis
Abstract
Lung cancer is one type of cancer with the high incidence rate in the world, and it is very important to find the best treatment therapy to reduce the problem of lung cancer. African leaves (Vernonia amygdalina Delile.) have long been used as medicine in the world of health. This study evaluated the anticancer activity of ethylacetate extract (EAE) of Vernonia amygdalina Delile. leaves towards HTB-182 cell line. EAE was analyzed for flavonoids constituent and tested for cytotoxicity, cell cycle inhibition, apoptosis induction and inhibition of PI3KCA, EGFR and VEGFR-2 genes expression. EAE was found to contain quercetin, quercetin 3-O--L-arabinopyranoside and isoquercetin. EAE of Vernonia amygdalina Delile. leaves were found to have IC50 of 10.37 0.16 g/mL, caused accumulation in S and G2-M phase, caused apoptosis and decreased PI3KCA, EGFR and VEGFR-2 genes expression. The results reveal that EAE of Vernonia amygdalina Delile. leaves have cytotoxic activities by inhibition of cell cycle. Our further study is to isolate compounds responsible for the cytotoxic activity.