Antimycobacterial screening of selected medicinal plants against Mycobacterium tuberculosis H<sub>37</sub>Rv using agar dilution method and the microplate resazurin assay
KhushbooDhirajlal Jethva, DharaRajendrakumar Bhatt, Maitreyi Zaveri
Abstract
Background: Rv. Methods: Eleven medicinal plants were selected on the basis of its literature survey, and three different extracts were prepared. Antimycobacterial activities were screened using two in vitro assays, namely agar dilution assay and microplate resazurin assay against M. tuberculosis H37Rv at different concentrations of prepared extracts. We analyzed minimal inhibition concentrations and percentage of inhibition of the used strain of Mycobacteria. Isoniazid was used as a standard anti-TB drug. Results: The results of this study showed that aqueous extracts four selected medicinal plants Ocimum sanctum, Adhatoda vasica, Leptadenia reticulata, and Cocculus hirsutus having minimum inhibitory concentration at 500 μg/ml, 500 μg/ml, 250 μg/ml, and 250 μg/ml, respectively, and O. sanctum (60.24%), A. vasica (62.89%), L. reticulata (74.26%), and C. hirsutus (81.67%) showed significant anti-TB activity against M. tuberculosis. Conclusion: This study helps society to found new anti-TB agents having better anti-TB activity with lesser or no side effects.