Fungal glycoside hydrolase family 44 xyloglucanases are restricted to the phylum Basidiomycota and show a distinct xyloglucan cleavage pattern
Peicheng Sun, Xinxin Li, Adiphol Dilokpimol, Bernard Henrissat, Ronald P. de Vries, Mirjam A. Kabel, Miia Mäkelä
Abstract
were heterologously produced and characterized. They exhibited XEG activity and displayed a hydrolytic cleavage pattern different from that observed in fungal XEGs from other GH families. Specifically, the fungal GH44 XEGs were not hindered by substitution of neighboring glucosyl units and generated various "XXXG-type," "GXXX(G)-type," and "XXX-type" oligosaccharides. Overall, these fungal GH44 XEGs represent a novel class of enzymes for plant biomass conversion and valorization.
Topics & Concepts
BasidiomycotaXyloglucanGlycoside hydrolaseCleavage (geology)PhylumAnthocyanidinBotanyAscomycotaChemistryBiologyStereochemistryBiochemistryEnzymePaleontologyCell wallGeneFracture (geology)AnthocyaninPolysaccharides and Plant Cell WallsMycorrhizal Fungi and Plant InteractionsFungal Biology and Applications