Ag NCs as a potent antibiofilm agent against pathogenic <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> and drug-resistant <i>Bacillus subtilis</i> by affecting chemotaxis and flagellar assembly pathway genes
Anirudh Singh, Komal Rani, Vibha Tandon, Amaresh Kumar Sahoo, Sintu Kumar Samanta
Abstract
. Ag NCs were able to both prevent and eradicate the biofilm formation very effectively. The antibiofilm activity can be significantly increased with α-amylase and/or DNase which degrade the structural components of biofilms. The antibiofilm activity of antibiotics gets considerably lowered due to poor penetration and the acidic microenvironment of biofilms. However, the potency of antibiotics gets significantly increased when applied with Ag NCs. Finally, RNA seq-based analysis has demonstrated that the biofilm degradation was likely due to the regulation of bacterial chemotaxis and flagellar assembly pathway genes by Ag NCs.
Topics & Concepts
Acinetobacter baumanniiPseudomonas aeruginosaBacillus subtilisMicrobiologyBiofilmChemotaxisChemistryBacteriaBiologyBiochemistryReceptorGeneticsNanocluster Synthesis and ApplicationsNanoparticles: synthesis and applicationsBacterial biofilms and quorum sensing