Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus.
Sara Torabi, Sina Soleimani, Homayoon Mahravani, Mohammad Majid Ebrahimi, Shahla Shahsavandi
Abstract
until the end passage. The IBDV replication in connection with DNA fragmentation and FITC, revealed the characteristic picture of apoptosis in a time-dependent manner. We found that the IBDV could easily be adapted to L929 cells, increasing virus yields by about two orders of magnitude. These results indicated that the cell line may be useful in the production of efficient virus particles.
Topics & Concepts
Infectious bursal diseaseVirusVirologyTiterBiologyMultiplicity of infectionViral replicationCytopathic effectCell cultureELISPOTDoubling timeVirulenceImmunologyT cellGeneImmune systemGeneticsVirology and Viral DiseasesVirus-based gene therapy researchVector-Borne Animal Diseases