Construction of a new thermophilic fungus Myceliophthora thermophila platform for enzyme production using a versatile 2A peptide strategy combined with efficient CRISPR-Cas9 system
Fangya Li, Qian Liu, Xiaolin Li, Chenyang Zhang, Jingen Li, Wenliang Sun, Dandan Liu, Dongguang Xiao, Chaoguang Tian
Topics & Concepts
CRISPRBiologyThermophileGreen fluorescent proteinHeterologousBiochemistryRecombinant DNAEnzymeGeneFungal and yeast genetics researchEnzyme Production and CharacterizationCRISPR and Genetic Engineering