Biodegradation of Aflatoxin B<sub>1</sub> in Peanut Oil by an Amphipathic Laccase–Inorganic Hybrid Nanoflower
Tianying Lu, Caicai Fu, Yonghua Xiong, Zheling Zeng, Yunkai Fan, Xiao Dai, Xiaolin Huang, Jun Ge, Xiaoyang Li
Abstract
Aflatoxin B 1 (AFB 1 ) contamination is an important issue for the safety of edible oils. Enzymatic degradation is a promising approach for removing mycotoxins in a specific, efficient, and green manner. However, enzymatic degradation of mycotoxins in edible oil is challenging as a result of the low activity and stability of the enzyme. Herein, a novel strategy was proposed to degrade AFB 1 in peanut oil using an amphipathic laccase–inorganic hybrid nanoflower (Lac NF–P) as a biocatalyst. Owing to the improved microenvironment of the enzymatic reaction and the enhanced stability of the enzyme structure, the proposed amphipathic Lac NF–P showed 134- and 3.2-fold increases in the degradation efficiency of AFB 1 in comparison to laccase and Lac NF, respectively. AFB 1 was removed to less than 0.96 μg/kg within 3 h when using Lac NF–P as a catalyst in the peanut oil, with the AFB 1 concentration ranging from 50 to 150 μg/kg. Moreover, the quality of the peanut oil had no obvious change, and no leakage of catalyst was observed after the treatment of Lac NF–P. In other words, our study may open an avenue for the development of a novel biocatalyst for the detoxification of mycotoxins in edible oils.