Functional Interaction Between GABAergic Neurons in the Ventral Tegmental Area and Serotonergic Neurons in the Dorsal Raphe Nucleus
Sheikh Mizanur Rahaman, Srikanta Chowdhury, Yasutaka Mukai, Daisuke Ono, Hiroshi Yamaguchi, Akihiro Yamanaka
Abstract
GABAergic neurons in the ventral tegmental area (VTA) have brain-wide projections and are involved in multiple behavioral and physiological functions. Here, we revealed the responsiveness of Gad67+ neurons in VTA (VTA Gad67+ ) to various neurotransmitters involved in the regulation of sleep/wakefulness by slice patch clamp recording. Among the substances tested, a cholinergic agonist activated, but serotonin, dopamine and histamine inhibited these neurons. Dense VTA Gad67+ neuronal projections were observed in brain areas regulating sleep/wakefulness, including the central amygdala (CeA), dorsal raphe nucleus (DRN), and locus coeruleus (LC). Using a combination of electrophysiology and optogenetic studies, we showed that VTA Gad67+ neurons inhibited all neurons recorded in the DRN, but did not inhibit randomly recorded neurons in the CeA and LC. Further examination revealed that the serotonergic neurons in the DRN (DRN 5–HT ) were monosynaptically innervated and inhibited by VTA Gad67+ neurons. All recorded DRN 5–HT neurons received inhibitory input from VTA Gad67+ neurons, while only one quarter of them received inhibitory input from local GABAergic neurons. Gad67+ neurons in the DRN (DRN Gad67+ ) also received monosynaptic inhibitory input from VTA Gad67+ neurons. Taken together, we found that VTA Gad67+ neurons were integrated in many inputs, and their output inhibits DRN 5–HT neurons, which may regulate physiological functions including sleep/wakefulness.