Mapping of wheat stripe rust resistance gene Yr041133 by BSR-Seq analysis
Yahui Li, Ruiming Lin, Jinghuang Hu, Xiaohan Shi, Dan Qiu, Peipei Wu, Gebremedhin Habteab Goitom, Siqi Wang, Hongjun Zhang, Li Yang, Hongwei Liu, Qiuhong Wu, Jingzhong Xie, Yang Zhou, Zhiyong Liu, Hongjie Li
Abstract
Puccinia striiformis Westend. f. sp. tritici (Pst) pathotype CYR34 is widely virulent and prevalent in China. Here, we report identification of a strpie rust resistance (Yr) gene, designated Yr041133, in winter wheat line 041133. This line produced a hypersensitive reaction to CYR34 and conferred resistance to 13 other pathotypes. Resistance to CYR34 in line 041133 was controlled by a single dominant gene. Bulked segregant RNA sequencing (BSR-Seq) was performed on a pair of RNA bulks generated by pooling resistant and susceptible recombinant inbred lines. Yr041133 was mapped to a 1.7 cM genetic interval on the chromosome arm 7BL that corresponded to a 0.8 Mb physical interval (608.9–609.7 Mb) in the Chinese Spring reference genome. Based on its unique physical location Yr041133 differred from the other Yr genes on this chromosome arm.